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Human EGF (Epidermal Growth Factor) Microsample ELISA Kit

CAT: 0855-ELK10240MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK10240MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
URG; Beta-Urogastrone; Pro-epidermal growth factor
UniProt
P01133
Reactivity
Human
Field of Research
Cytokine; Tumor immunity; Infection immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EGF in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
2000 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
31.25-2000 pg/mL
Sensitivity
7.66 pg/mL

UniProtKB · P01133

Pro-epidermal growth factor

EGF_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P01133
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
EGF
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
EGF
EC number
—
Processing
Precursor
Secondary accessions
B4DRK7, E7EVD2, E9PBF0, Q52LZ6
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Disease

Disease variantPrimary hypomagnesemia

PTM

Disulfide bondGlycoprotein

Domain

EGF-like domainRepeatSignalTransmembraneTransmembrane helix

Molecular function

Growth factor

Cellular component

Membrane

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