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Human PLGF (Placenta Growth Factor) Microsample ELISA Kit

CAT: 0855-ELK1178MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK1178MS-01Size:48 Tests
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Product Name Alternative
PlGF2; PGF; PGFL; Placental Growth Factor-Like; Vascular Endothelial Growth Factor-Related Protein
UniProt
P49763
Reactivity
Human
Field of Research
Cytokine; Reproductive science
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PLGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PLGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PLGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PLGF in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
2000 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
31.25-2000 pg/mL
Sensitivity
7.8 pg/mL

UniProtKB · P49763

Placenta growth factor

PLGF_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P49763
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PGF
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
PlGF
EC number
—
Processing
Precursor
Secondary accessions
Q07101, Q9BV78, Q9Y6S8
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

AngiogenesisDifferentiation

Molecular function

Developmental proteinGrowth factorHeparin-bindingMitogen

PTM

Disulfide bondGlycoprotein

Cellular component

Secreted

Domain

Signal

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