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Rat NGF (Nerve Growth Factor) Microsample ELISA Kit

CAT: 0013-GTR18872992-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872992-01Size:48 Tests
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Description
Rat NGF (Nerve Growth Factor) Microsample ELISA Kit
Product Name Alternative
NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor
UniProt
P25427
Reactivity
Rat
Field of Research
Neuroscience; Stem Cell & Developmental Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
6.1 pg/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NGF in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
15.63-1000 pg/mL

UniProtKB · P25427

Beta-nerve growth factor

NGF_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P25427
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Ngf
Protein existence
2: Evidence at transcript level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
Beta-NGF
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoprotein

Cellular component

EndosomeSecreted

Molecular function

Growth factorMetalloenzyme inhibitorMetalloprotease inhibitorProtease inhibitor

Ligand

Lipid-binding

Technical term

Reference proteome

Domain

Signal

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