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Human NGF (Nerve Growth Factor) Microsample ELISA Kit

CAT: 0855-ELK1173MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK1173MS-01Size:48 Tests
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Product Name Alternative
NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor
UniProt
P01138
Reactivity
Human
Field of Research
Cytokine; Neuro science
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NGF in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
1000 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
15.63-1000 pg/mL
Sensitivity
6.2 pg/mL

UniProtKB · P01138

Beta-nerve growth factor

NGF_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P01138
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
NGF
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
Beta-NGF
EC number
—
Processing
Precursor
Secondary accessions
A1A4E5, Q6FHA0, Q96P60, Q9P2Q8, Q9UKL8
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoprotein

Disease

Disease variantNeurodegenerationNeuropathy

Cellular component

EndosomeSecreted

Molecular function

Growth factorMetalloenzyme inhibitorMetalloprotease inhibitorProtease inhibitor

Ligand

Lipid-binding

Domain

Signal

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