Human C4a (Complement Component 4a) ELISA Kit

CAT: 0855-ELK4951-01Size: 48 TestsDry Ice: NoHazardous: No
CAT#:0855-ELK4951-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C4a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C4a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C4a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C4a in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
C4a anaphylatoxin
UniProt
P0C0L4
Reactivity
Human
Field of Research
Immune molecule; Endocrinology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C4a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C4a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C4a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C4a in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.151 ng/mL

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