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Rat C5a (Complement Component 5a) Microsample ELISA Kit

CAT: 0855-ELK1593MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK1593MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
Complement Component 5a
UniProt
P08650
Reactivity
Rat
Field of Research
Infection immunity; Immune molecule; Autoimmunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat C5a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat C5a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat C5a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat C5a in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
100 ng/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
1.57-100 ng/mL
Sensitivity
0.63 ng/mL

UniProtKB · P08650

Complement C5

CO5_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P08650
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
C5
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A0A096P6L9, Q63078
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoprotein

Biological process

Complement alternate pathwayComplement pathwayCytolysisImmunityInflammatory responseInnate immunity

Cellular component

MembraneMembrane attack complexSecretedTarget cell membraneTarget membrane

Domain

Signal

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