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Mouse OC (Osteocalcin) ELISA Kit

CAT: 0855-ELK11106-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK11106-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse OC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse OC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse OC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse OC in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein
UniProt
P86546
Reactivity
Mouse
Field of Research
Metabolic pathway; Endocrinology; Hormone metabolism; Bone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse OC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse OC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse OC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse OC in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
100 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
1.57-100 ng/mL
Sensitivity
0.61 ng/mL

UniProtKB · P86546

Osteocalcin

OSTCN_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P86546
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Bglap
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
OG1
EC number
—
Processing
Precursor
Secondary accessions
P04641
Protein keywords

Biological process

Biomineralization

Ligand

CalciumMetal-binding

PTM

Cleavage on pair of basic residuesDisulfide bondGamma-carboxyglutamic acid

Molecular function

Hormone

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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