Products for Research Use Only
RegisterLogin

EasyStep Human OC (Osteocalcin) ELISA Kit

CAT: 0855-ELK009ES-01Size: 48 TestsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0855-ELK009ES-01Size:48 Tests
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OC, and the Human OC standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human OC. After TMB substrate solution is added, only those wells that contain Human OC and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OC in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein
UniProt
P02818
Reactivity
Human
Field of Research
Metabolic pathway; Endocrinology; Hormone metabolism; Bone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OC, and the Human OC standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells , then added a HRP-conjugated antibody specific to Human OC. After TMB substrate solution is added, only those wells that contain Human OC and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OC in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
1.5h
Standard
15 ng/mL
Sample Type
Serum
Detection Range
0.23-15 ng/mL
Sensitivity
0.14 ng/mL

UniProtKB · P02818

Osteocalcin

OSTCN_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P02818
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
BGLAP
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q5TCK6
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Biological process

Biomineralization

Ligand

CalciumMetal-binding

PTM

Cleavage on pair of basic residuesDisulfide bondGamma-carboxyglutamic acid

Molecular function

Hormone

Cellular component

Secreted

Domain

Signal

Related Products

CatalogName
ELK2390-01Human OC (Osteocalcin) ELISA Kit
ELK2390MS-01Human OC (Osteocalcin) Microsample ELISA Kit
ELK11106-01Mouse OC (Osteocalcin) ELISA Kit
ELK0003-01Goat OC (Osteocalcin) ELISA Kit
ELK5730-01Pig OC (Osteocalcin) ELISA Kit
ELK11112-01Rabbit OC (Osteocalcin) ELISA Kit
ELK10607-01Chicken OC (Osteocalcin) ELISA Kit
ELK7628-01Dog OC (Osteocalcin) ELISA Kit
ELK11144-01Cattle OC (Osteocalcin) ELISA Kit
ELK2391-01Rat OC (Osteocalcin) ELISA Kit

Alternative Products

CatalogName
MBS8820576-01EasyStep Human OC (Osteocalcin) ELISA Kit
GTR18856046-01Human Osteocalcin (OC) EasyStep ELISA Kit
MBS8821051-01EasyStep Human TRF (Transferrin) ELISA Kit
MBS8821019-01EasyStep Human OPG (Osteoprotegerin) ELISA Kit
RDE-PCT-HuEASYStep Human Procalcitonin (PCT) ELISA Kit
RDE-IgA-HuEASYStep Human Immunoglobulin A (IgA) ELISA Kit
RDE-ADP-HuEASYStep Human Adiponectin (ADP) ELISA Kit
GTR18859405-01Human Estradiol (E2) EasyStep ELISA Kit
GTR18859303-01Human Adiponectin (ADP) EasyStep ELISA Kit
GTR18859261-01Human Osteoprotegerin (OPG) EasyStep ELISA Kit

Popular Products