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Pig OC (Osteocalcin) ELISA Kit

CAT: 0855-ELK5730-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK5730-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig OC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig OC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig OC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig OC in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein
UniProt
Q8HYY9
Reactivity
Pig
Field of Research
Metabolic pathway; Endocrinology; Hormone metabolism; Bone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig OC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig OC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig OC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig OC in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.122 ng/mL

UniProtKB · Q8HYY9

Osteocalcin

OSTCN_PIG · Sus scrofa

View on UniProt ↗
Primary accession
Q8HYY9
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
BGLAP
Protein existence
1: Evidence at protein level
Organism
Sus scrofa (Pig)
Taxonomy ID
9823
Alternative names
—
EC number
—
Processing
—
Secondary accessions
—
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Biological process

Biomineralization

Ligand

CalciumMetal-binding

PTM

Disulfide bondGamma-carboxyglutamic acidHydroxylation

Molecular function

Hormone

Cellular component

Secreted

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