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Rat OC (Osteocalcin) ELISA Kit

CAT: 0855-ELK2391-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2391-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat OC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat OC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat OC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat OC in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein
UniProt
P04640
Reactivity
Rat
Field of Research
Metabolic pathway; Endocrinology; Hormone metabolism; Bone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat OC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat OC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat OC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat OC in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
50 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Detection Range
0.78-50 ng/mL
Sensitivity
0.29 ng/mL

UniProtKB · P04640

Osteocalcin

OSTCN_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P04640
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Bglap
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Biological process

Biomineralization

Ligand

CalciumMetal-binding

PTM

Cleavage on pair of basic residuesDisulfide bondGamma-carboxyglutamic acidHydroxylation

Molecular function

Hormone

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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