Recombinant Mouse GITR/TNFRSF18 Protein (Fc Tag)

• Background:

  GITR, also known as TNFRSF18 (CD357), belongs to the tumor necrosis factor receptor (TNF-R) superfamily. It is the receptor for TNFSF18. GITR plays a key role in dominant immunological self-tolerance maintained by CD25 (+) CD4 (+) regulatory T cells. GITR may be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. GITR and its ligand are important costimulatory molecules in the pathogenesis of autoimmune diseases. It also mediates NF-kappa-B activation via the TRAF2/NIK pathway.

• Abbreviation:

  GITR/TNFRSF18

• Synonyms:

  Tumor necrosis factor receptor superfamily member; Tnfrsf; AITR; CD357; GITR-D; Glucocorticoid-induced TNFR-related protein; Tumor necrosis factor receptor superfamily member 18; Gitr; Tnfrsf18; GITR; AITR

• UniProt:

  O35714

• Accession Number:

  O35714

• Expression System:

  Mammalian

• Tag:

  C-Fc

• Sequence:

  Gln20-His153

• Endotoxin:

  < 1.0 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 90% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  39.6 kDa

• Observed Molecular Weight:

  50 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

• Species:

  Mouse