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T7 RNA polymerase

CAT: 0804-HY-E70090-01Size: 1 KUDry Ice: NoHazardous: No
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CAT#:0804-HY-E70090-01Size:1 KU
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
T7 RNA polymerase is a polymerase expressed by Escherichia coli from the RNA polymerase gene of T7 bacteriophage. T7 RNA polymerase is highly specific and involved in in vitro transcription (IVT) of mRNA. In the presence of Mg2+, T7 RNA polymerase only uses the single-stranded or double-stranded DNA containing the T7 promoter sequence as a template, and uses NTP as a substrate to synthesize RNA complementary to the single-stranded DNA downstream of the promoter[1][2].
CAS Number
9014-24-8
UNSPSC
12352204
Target
DNA/RNA Synthesis
Type
Enzyme
Related Pathways
Cell Cycle/DNA Damage
Applications
Cancer-programmed cell death
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/t7-rna-polymerase.html
Purity
0
Solubility
10 mM in DMSO
Smiles
[T7RNApolymerase]
References & Citations
[1]Dousis A, et al. An engineered T7 RNA polymerase that produces mRNA free of immunostimulatory byproducts. Nat Biotechnol. 2023 Apr;41 (4) :560-568. |[2]Borkotoky S, et al. The highly efficient T7 RNA polymerase: A wonder macromolecule in biological realm. Int J Biol Macromol. 2018 Oct 15;118 (Pt A) :49-56.
Shipping Conditions
Blue Ice
Storage Conditions
Store at -20°C for 2 years
Scientific Category
Enzyme
Clinical Information
No Development Reported

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