Anti-SNCA | Alpha-synuclein

• Background :

  Alpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy.

• CAS Number :

  9007-83-4

• HS Code :

  38221900

• Gene ID :

  6622

• UniProt :

  P37840

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  Recombinant, full length, human alpha-synuclein UniProt: P37840, epitope was mapped between amino acid 1-15

• Clonality :

  Polyclonal

• Applications :

  ELISA (ELISA), Immunolocalization (IL), Western blot (WB)

• Field of Research :

  Parkinson's disease

• Dilution :

  1 : 3000 (ELISA), 1-2 µg/ml (IL), 1 : 1000 (WB)

• Purity :

  Serum

• Format :

  Lyophilized

• Reconstitution :

  For reconstitution add 100 µl of sterile water

• Molecular Weight :

  14 kDa

• Additionnal Information :

  Immunolocalization: human tissue was paraffin-embedded and sectioned. De-waxed and rehydrated in an ethanol gradient. Antigens were retrieved in sodium citrate buffer (pH 6) at 95°C for 1 h. The tissue sections were separately incubated for 1 h at RT with primary antibody and antibody binding was visualized with IgG Preoxidase Reagent Kit.This antibody will recognize human SNCA monomers and multimers in Western blot and can be used to detect fibrills in a sandwich ELISA. In ELISA this antibody can be used for detection, combined with AS13 2719 as a capture antibody.

• References & Citations :

  Bargar et al. (2021) Discrimination of MSA-P and MSA-C by RT-QuIC analysis of olfactory mucosa: the first assessment of assay reproducibility between two specialized laboratories. Mol Neurodegener. 2021 Dec 11;16 (1) :82. doi: 10.1186/s13024-021-00491-y. PMID: 34895275; PMCID: PMC8665327.Brännström et al. (2014) . A generic method for design of oligomer-specific antibodies. PLoS One. 2014 Mar 11;9 (3) :e90857. doi: 10.1371/journal.pone.0090857. eCollection 2014.

• Storage Conditions :

  Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• Prediction Reactivity :

  Primates, Chimpanzee, Gorilla