Anti-ASyO5 | Mouse anti-human alpha-synuclein | oligomer-specific (clone number 2,4)

Anti-ASyO5 | Mouse anti-human alpha-synuclein | oligomer-specific (clone number 2,4)

• Background: Alpha-synucleinis normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy. In analogy to many other amyloid associated disorders, alpha-synuclein may also form oligomeric assemblies. These small and soluble forms have been suggested to exert a stronger tissue damaging effect as compared to the monomeric and fibrillar counterpart. Using a recently developed technique a monoclonal oligomer-specific antibody has been designed.
• CAS Number: 9007-83-4
• Host: Mouse
• Reactivity: Human, mouse
• Immunogen: Synthetic peptide derived from human alpha-synuclein Gly111-Tyr125
• Clonality: Monoclonal
• Applications: Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC)
• Dilution: 1-2 µg/mL (Dot), 2-4 µg/mL (ELISA capture), 10 µg/mL (IHC)
• Purity: Immunogen affinity purified serum in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 14 kDa
• References & Citations: Kilpeläinenet al (2023) Nonpeptidic Oxazole-Based Prolyl Oligopeptidase Ligands with Disease-Modifying Effects on α-Synuclein Mouse Models of Parkinson's Disease.Etelainenet al. (2022) Removal of proteinase K resistant alfaSyn species does not correlate with cell survival in a virus vector-based Parkinson's disease mouse model. Neuropharmacology. 2022;218:109213. doi:10.1016/j.neuropharm.2022.109213.Hoet al. (2022). LRRK2 Inhibition Mitigates the Neuroinflammation Caused by TLR2-Specific alfa-Synuclein and Alleviates Neuroinflammation-Derived Dopaminergic Neuronal Loss. Cells. 2022 Mar 2;11(5):861. doi: 10.3390/cells11050861. PMID: 35269482; PMCID: PMC8909553.Etelainen, Kilpelainen, Ignatius, et al. (2022) Removal of proteinase K resistant αSyn species does not correlate with cell survival in a virus vector-based Parkinson's disease mouse model. Neuropharmacology. 2022;218:109213. doi:10.1016/j.neuropharm.2022.109213.Limegroveret al. (2021) Sigma-2 receptor antagonists rescue neuronal dysfunction induced by Parkinson's patient brain-derived a-synuclein. J Neurosci Res. 2021 Apr;99(4):1161-1176. doi: 10.1002/jnr.24782. Epub 2021 Jan 22. PMID: 33480104.Kilpeläinenet al. (2019). Behavioural and dopaminergic changes in double mutated human A30P*A53T alpha-synuclein transgenic mouse model of Parkinson´s disease.Sci Rep. 2019 Nov 22;9(1):17382. doi: 10.1038/s41598-019-54034-z.Wuet al. (2017). The critical role of Nramp1 in degrading a-synuclein oligomers in microglia under iron overload condition. Neurobiol Dis. 2017 Aug;104:61-72. doi: 10.1016/j.nbd.2017.05.001. (human, mouse, immunolocalization)Svarcbahset al. (2016). Inhibition of Prolyl Oligopeptidase Restores Spontaneous Motor Behavior in the a-Synuclein Virus Vector-Based Parkinson's Disease Mouse Model by Decreasing a-Synuclein Oligomeric Species in Mouse Brain. J Neurosci. 2016 Dec 7;36(49):12485-12497.Brännströmet al. (2014). A Generic Method for Design of Oligomer-Specific Antibodies. PLoS ONE. DOI: 10.1371/journal.pone.0090857.
• Storage Conditions: For short time storage add sodium azide and store at +4°C. For long time storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.