Mouse CXCL10/IP-10 ELISA Kit PicoKine®, Carrier-free

• Background:

  Chemokine (C-X-C motif) ligand 10 (CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family that is also known as 10 kDa interferon-gamma-induced protein (gamma-IP10 or IP-10). CXCL10 is secreted by several cell types in response to IFN-gamma. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.It is a potent inhibitor of angiogenesis in vivo. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. It is a RAS target gene and is overexpressed in the majority of colorectal cancers.This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.

• :

  heparin

• Description:

  Mouse CXCL10/IP-10 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cxcl10 in cell culture supernatants, serum and plasma ( heparin). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	105	98	104	102	102	2.68	2.6%
  Sample 2	245	274	275	259	263	12.29	4.6%
  Sample 3	923	908	845	862	884	32.01	3.6%

  *number of samples for each test n=16.

• Synonyms:

  C-X-C motif chemokine 10;10 kDa interferon gamma-induced protein;Gamma-IP10;IP-10;C7;Interferon-gamma induced protein CRG-2;Small-inducible cytokine B10;Cxcl10;Crg2, Ifi10, Inp10, Scyb10;

• Gene Name:

  C-X-C motif chemokine 10

• Gene ID:

  15945

• UniProt:

  P17515

• Reactivity:

  Mouse

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: E.coli; Immunogen sequence: I22-P98

• Tissue Specificity:

  Expressed in the spleen, thymus, lymph nodes and liver. .

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, serum and plasma ( heparin).

• Detection Range:

  31.2 pg/ml - 2,000 pg/ml

• Sensitivity:

  <1 pg/ml

• Function:

  In addition to its role as a proinflammatory cytokine, may participate in T-cell effector function and perhaps T-cell development.

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  10789 MW

• Specificity:

  Natural and recombinant mouse CXCL10

• Subcellular Location:

  Secreted.

• Protein Name:

  C-X-C motif chemokine 10