Recombinant Rabbit TNF-alpha/TNFSF2/TNFa Protein

• Background:

  Tumor necrosis factor alpha (TNFα) is the prototypic ligand of the TNF superfamily. TNFα forms a homotrimer and functions by activating two types of receptors TNF-R1 (TNF receptor type 1, p55R) and TNF-R2 (TNF receptor type 2, p75R) . TNFα is a pleiotropic cytokine that is capable to promote inflammation, to induce apoptotic cell death, and to inhibit tumorigenesis and viral replication. TNFα is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.

• Abbreviation:

  TNF-alpha; TNFSF2; TNFa

• Synonyms:

  Cachectin; TNF; TNF-Alpha; TNF-a; TNFA; TNFSF2; Tumor Necrosis Factor; Tumor Necrosis Factor Ligand Superfamily Member 2

• UniProt:

  P04924

• Accession Number:

  P04924

• Expression System:

  E.coli

• Tag:

  None

• Sequence:

  Val78-Leu235

• Applications:

  Cell culture

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 3. 5 pg/ml.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  17.6 kDa

• Observed Molecular Weight:

  18 kDa

• Species:

  Rabbit