Recombinant Mouse TNF-alpha/TNFA Protein

Recombinant Mouse TNF-alpha/TNFA Protein

• Background: Tumor Necrosis Factor (TNF) is a member of the Tumor Necrosis Factor family. TNF exists as a homotrimer and interacts with SPPL2B. TNF is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes throught its effects on insulin resistance and fatty acid metabolism.
• Abbreviation: TNF-alpha;TNFA
• Synonyms: Cachectin, DIF, Membrane Form, TNF-Alpha, TNF-a, TNF-alpha, TNFSF2, TNFalpha, Tnf, Tnfa, Tnfsf1a, Tnfsf2, Tumor Necrosis Factor, Tumor Necrosis Factor Ligand Superfamily Member 2
• CAS Number: 9000-83-3
• UniProt: P06804
• Accession Number: P06804
• Host: E. coli
• Origin Species: Mouse
• Tag: None
• Sequence: Asp89-Leu235
• Applications: Cell culture
• Purity: > 95 % as determined by reducing SDS-PAGE.
• Bioactivity: Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 2-8 pg/ml.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 14 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.