Recombinant Rat TNF-alpha/TNFA Protein

Recombinant Rat TNF-alpha/TNFA Protein

• Background: Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.
• Synonyms: MGC124630, RATTNF, TNF-alpha, Tnf, Tnfa
• CAS Number: 9000-83-3
• UniProt: P16599
• Accession Number: P16599
• Host: E. coli
• Origin Species: Rat
• Tag: None
• Sequence: Leu 80-Leu 235
• Applications: Cell culture
• Purity: > 93 % as determined by reducing SDS-PAGE.
• Bioactivity: Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 20-80 pg/ml.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 15 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.