Recombinant Mouse TNF-alpha/TNFA Protein (aa 80-235)

• Background:

  Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1) . TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment. TNF-alpha Protein & Antibody

• Abbreviation:

  TNF-alpha, TNFA

• Synonyms:

  Cachectin; DIF; Membrane Form; TNF-Alpha; TNF-a; TNF-alpha; TNFSF2; TNFalpha; Tnf; Tnfa; Tnfsf1a; Tnfsf2; Tumor Necrosis Factor; Tumor Necrosis Factor Ligand Superfamily Member 2

• UniProt:

  P06804

• Accession Number:

  P06804

• Expression System:

  E. coli

• Tag:

  C-His

• Sequence:

  Leu 80-Leu 235

• Applications:

  Cell culture

• Endotoxin:

  < 0.1 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 98 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED₅₀ for this effect is <40 pg/mL. The specific activity of recombinant mouse TNF alpha is approximately >2.5x 10⁷ IU/mg.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  18.2 kDa

• Observed Molecular Weight:

  16 kDa

• Formulation:

  Lyophilized from sterile PBS, pH 8.0.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Mouse