Recombinant Mouse GM-CSF/CSF2 Protein (His Tag)

Recombinant Mouse GM-CSF/CSF2 Protein (His Tag)

• Background: Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factorthat can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by anumber of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cellsand fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophageprogenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. Onmature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has a functional role on nonhematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF canalso stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma andadenocarcinoma cell lines.
• Abbreviation: GM-CSF;CSF2
• Synonyms: CSF, CSF2, Colony-Stimulating Factor, GM-CSF, GMCSF, Granulocyte-Macrophage Colony-Stimulating Factor, Molgramostin, Sargramostim
• CAS Number: 9000-83-3
• UniProt: P01587
• Accession Number: P01587
• Host: HEK293 Cells
• Origin Species: Mouse
• Tag: C-His
• Sequence: Ala18-Lys141
• Applications: Cell culture
• Purity: > 95 % as determined by reducing SDS-PAGE.
• Bioactivity: Measured in a cell proliferation assay using PDC-P1 cells. The ED50 for this effect is 40-170 pg/ml.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 18-30 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.