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Recombinant Mouse GM-CSF (CSF2)

CAT: 0171-Z200077Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0171-Z200077Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
GM-CSF is a monomeric cytokine with two glycosylation sites that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. CSF2 is secreted together with other factors by T cells and macrophages following cell activation by antigens or mitogens. The signalling ‘GM-CSF:receptor’ complex is a dodecamer of two hexamers of two alpha, two beta, and two ligand subunits. While non-glycosylated and glycosylated GM-CSF show similar activities in vitro, fully glycosylated GM-CSF is biologically more active in vivo. Recombinant Mouse CSF2 is a non-glycosylated, monomeric protein.
CAS Number
9000-83-3
Gene Name
CSF2
Gene Aliases
GMCSF, Granulocyte-macrophage colony-stimulating factor, CSF2
Recombinant Mouse Granulocyte Macrophage Colony Stimulating Factor (CSF2)
Gene ID
12981
Accession Number
P01587
Organism
Mouse
Source
E. coli
Concentration
< 1.0 EU/μg of recombinant protein as determined by the LAL method.
Purity
>95% as determined by SDS-PAGE
Solubility
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Function
The ED50 as determined by the dose-dependent stimulation BaF3 cells was < 0.1 ng/mL
Molecular Weight
14
Shipping Conditions
Ambient Temperature
Storage Conditions
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Appearance
Lyophilized Powder
Formulation
Lyophilized from 0.2 μm filtered solution in Tris, pH 7.5 and NaCl

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