Recombinant Human GM-CSF/CSF2 Protein (His Tag, Cells)

• Background:

  Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells; B cells; macrophages; mast cells; endothelial cells and fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors; GM-CSF is also a growth factor for erythroid; megakaryocyte and eosinophil progenitors. On mature hematopoietic; monocytes/ macrophages and eosinophils. GM-CSF has a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally; GM-CSF can also stimulate the proliferation of a number of tumor cell lines; including osteogenic sarcoma; carcinoma and adenocarcinoma cell lines.

• Abbreviation:

  GM-CSF; CSF2

• Synonyms:

  CSF; CSF2; Colony-Stimulating Factor; GM-CSF; GMCSF; Granulocyte-Macrophage Colony-Stimulating Factor; Molgramostin; Sargramostim

• UniProt:

  P04141

• Accession Number:

  P04141

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Sequence:

  Ala18-Glu144

• Applications:

  Cell culture

• Field of Research:

  Immunology

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 6-30 pg/ml.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  15.5 kDa

• Observed Molecular Weight:

  17-30 kDa

• Species:

  Human