Recombinant Human GM-CSF/CSF2 Protein (HEK293 Cells)

Recombinant Human GM-CSF/CSF2 Protein (HEK293 Cells)

• Background: Granulocyte-macrophage colony-stimulating factor (GM-CSF) is one of an array of cytokines with pivotal roles in embryo implantation and subsequent development. Several cell lineages in the reproductive tract and gestational tissues synthesise GM-CSF under direction by ovarian steroid hormones and signalling agents originating in male seminal fluid and the conceptus. The pre-implantation embryo, invading placental trophoblast cells and the abundant populations of leukocytes controlling maternal immune tolerance are all subject to GM-CSF regulation. GM-CSF stimulates the differentiation of hematopoietic progenitors to monocytes and neutrophils, and reduces the risk for febrile neutropenia in cancer patients. GM-CSF also has been shown to induce the differentiation of myeloid dendritic cells (DCs) that promote the development of T-helper type 1 immune responses in cognate T cells. As a part of the immune/inflammatory cascade, GM-CSF promotes Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity, and thus worthy of consideration for therapeutic target. GM-CSF has been utilized in the clinical management of multiple disease processes. Most recently, GM-CSF has been incorporated into the treatment of malignancies as a sole therapy, as well as a vaccine adjuvant. While the benefits of GM-CSF in this arena have been promising, recent reports have suggested the potential for GM-CSF to induce immune suppression and, thus, negatively impact outcomes in the management of cancer patients.
• Synonyms: CSF, CSF2, Colony-Stimulating Factor, GM-CSF, GMCSF, Granulocyte-Macrophage Colony-Stimulating Factor, Molgramostin, Sargramostim
• CAS Number: 9000-83-3
• UniProt: P04141
• Accession Number: NP_000749.2
• Host: HEK293 Cells
• Origin Species: Human
• Tag: None
• Sequence: Met 1-Glu144
• Applications: Cell culture
• Field of Research: immunology
• Purity: > 90 % as determined by reducing SDS-PAGE.
• Bioactivity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.6 ng/ml.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 23.8 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.