Products for Research Use Only

Recombinant Human FGF-2/FGF basic/FGFb Protein

CAT: 0763-PKSH033503-01Size: 10 µgDry Ice: NoHazardous: No
Product image 1Product image 2Product image 3
1 / 3
CAT#:0763-PKSH033503-01Size:10 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Background
Fibroblast growth factors (FGF) are a family of heparin-binding secreted proteins that stimulate cell proliferation and differentiation in a wide variety of tissues. FGFs play important roles in diverse biological functions both in vivo and in vitro; including mitogenesis; cellular migration; differentiation; angiogenesis; and wound healing. Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state. Thermostable FGF basic was engineered for enhanced stability in culture media; without modification of its biological function. FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state. Because FGF basic is unstable; daily media changes are needed. The thermostable FGF basic that supports a 2-day media change schedule; so no media changes are required over a weekend. This thermostable FGF basic was more stable than FGF basic in biochemical studies; and maintained cell growth; pluripotency and differentiation potential with a 2-day feeding schedule.
Abbreviation
FGF-2; FGF basic; FGFb
Synonyms
Basic fibroblast growth factor; FGF-2; FGF2; FGFB; Fibroblast growth factor 2; HBGF-2; Heparin-binding growth factor 2; bFGF
UniProt
P09038
Accession Number
BAG70135.1
Expression System
E.coli
Tag
None
Sequence
Met1-Ser155
Field of Research
Cardiovascular, Signal Transduction, Neuroscience, Stem Cells, Cancer, Developmental Biology
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
17.2 kDa
Observed Molecular Weight
17 kDa
Formulation
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM NaCl, pH 7.5.
Species
Human
Product Datasheet
Loading...