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Recombinant human FGF basic/FGF-2 protein

CAT: 0112-ATGP4117-100Size: 100 µgDry Ice: NoHazardous: No
CAT#:0112-ATGP4117-100Size:100 µg
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Fibroblast growth factor 2 (FGF2) is a member of the FGF family that binds heparin and plays important roles in morphogenic, neurotrophic and angiogenic processes. FGF2 possess diverse biological functions, such as neuron differentiation, embryonic development and differentiation, modulation of angiogenesis, and wound healing. Recombinant human FGF2 was expressed in E. coli and purified by conventional chromatography techniques.
Specifications
Human FGF-2 stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cells in the presence of 10 µg/mL of heparin. The ED50 range ≤ to 0.2 ng/mL.
Product Name Alternative
Fibroblast growth factor 2, BFGF, FGFB, HBGF-2, FGF2, FGF-2
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells in the presence of 10 µg/mL of heparin. The ED50 range ≤ 0.2 ng/mL.
Molecular Weight
16.5 kDa (147aa) confirmed by MALDI-TOF
Additionnal Information
FGF basic, Fibroblast growth factor 2, BFGF, FGFB, HBGF-2, ATGP4117-10 µg, ATGP4117-20 µg, ATGP4117-50 µg, ATGP4117-100 µg, ATGP4117-250 µg, ATGP4117-500 µg, ATGP4117-1 mg, ATGP4117-10, ATGP4117-20, ATGP4117-50, ATGP4117-100, ATGP4117-250, ATGP4117-500, ATGP4117-1
References & Citations
Nakayama T., et al. (2007) Blood. 109 (2) :1363-72. ; ; A E Eriksson., et al. (1991) PNAS. 88:3441-3445; ; Raines, E.W. et al. (1985) Methods Enzymol. 109:749-73
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 50 mM Tris-HCl buffer (pH 8.0)
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_001997.5
Uniprot Accession Number
P09038
Species
Human
AA Sequence
MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS

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