Recombinant Human FGF-2/FGF basic/FGFb Protein (aa 1-155) (Q65I, C96S, N111G)

• Background:

  Fibroblast growth factors (FGF) are a family of heparin-binding secreted proteins that stimulate cell proliferation and differentiation in a wide variety of tissues. FGFs play important roles in diverse biological functions both in vivo and in vitro; including mitogenesis; cellular migration; differentiation; angiogenesis; and wound healing. Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state. Thermostable FGF basic was engineered for enhanced stability in culture media; without modification of its biological function. FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state. Because FGF basic is unstable; daily media changes are needed. The thermostable FGF basic that supports a 2-day media change schedule; so no media changes are required over a weekend. This thermostable FGF basic was more stable than FGF basic in biochemical studies; and maintained cell growth; pluripotency and differentiation potential with a 2-day feeding schedule.

• Abbreviation:

  FGF-2, FGF basic, FGFb

• Synonyms:

  Basic fibroblast growth factor; FGF-2; FGF2; FGFB; Fibroblast growth factor 2; HBGF-2; Heparin-binding growth factor 2; bFGF

• UniProt:

  P09038

• Accession Number:

  BAG70264.1

• Expression System:

  E. coli

• Tag:

  None

• Sequence:

  Met1-Ser155

• Field of Research:

  Cardiovascular, Signal Transduction, Neuroscience, Stem Cells, Cancer, Developmental Biology

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  17.2 kDa

• Observed Molecular Weight:

  16 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of 20mM Tris, 5% sucrose, 0.02% Tween 80, pH 8.0.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human