Recombinant Human FGF-2/FGF basic/FGFb Protein (aa 1-155)(Q65I,C96S,N111G)
CAT:
763-PKSH033502-02
Size:
10 µg
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No




Recombinant Human FGF-2/FGF basic/FGFb Protein (aa 1-155)(Q65I,C96S,N111G)
- Background: Fibroblast growth factors (FGF) are a family of heparin-binding secreted proteins that stimulate cell proliferation and differentiation in a wide variety of tissues. FGFs play important roles in diverse biological functions both in vivo and in vitro; including mitogenesis; cellular migration; differentiation; angiogenesis; and wound healing. Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state. Thermostable FGF basic was engineered for enhanced stability in culture media; without modification of its biological function. FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state. Because FGF basic is unstable; daily media changes are needed. The thermostable FGF basic that supports a 2-day media change schedule; so no media changes are required over a weekend. This thermostable FGF basic was more stable than FGF basic in biochemical studies; and maintained cell growth; pluripotency and differentiation potential with a 2-day feeding schedule.
- Abbreviation: FGF-2;FGF basic;FGFb
- Synonyms: Basic fibroblast growth factor, FGF-2, FGF2, FGFB, Fibroblast growth factor 2, HBGF-2, Heparin-binding growth factor 2, bFGF
- CAS Number: 9000-83-3
- UniProt: P09038
- Accession Number: BAG70264.1
- Host: E. coli
- Origin Species: Human
- Tag: None
- Sequence: Met1-Ser155
- Field of Research: Cardiovascular,Signal Transduction,Neuroscience,Stem Cells,Cancer,Developmental Biology
- Purity: > 95 % as determined by reducing SDS-PAGE.
- Bioactivity: Not validated for activity
- Reconstitution: Please refer to the printed manual for detailed information.
- Molecular Weight: 16 kDa
- Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
- Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.