Recombinant Human β-NGF/NGFB Protein

Recombinant Human β-NGF/NGFB Protein

• Background: Human β-Nerve Growth Factor (β-NGF) was initially isolated in the mouse submandibular gland. It is composed of three non-covalently linked subunits α; β; and γ; it exhibits all the biological activities ascribed to NGF. It is structurally related to BDNF; NT-3 and NT-4 and belongs to the cysteine-knot family of growth factors that assume stable dimeric structures. Β-NGF is a neurotrophic factor that signals through its receptor β-NGF; and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. These results suggest that β-NGF is a pleiotropic cytokine; which in addition to its neurotropic activities may have an important role in the regulation of the immune system. Human β-NGF shares 90% sequence similarity with mouse protein and shows cross-species reactivity.
• Abbreviation: β-NGF;NGFB
• Synonyms: Beta-NGF, Beta-Nerve Growth Factor, NGF, NGFB
• CAS Number: 9000-83-3
• UniProt: P01138
• Accession Number: P01138
• Host: E. coli
• Origin Species: Human
• Tag: C-His
• Sequence: Ser122-Ala241
• Applications: Cell culture
• Field of Research: Neuroscience
• Purity: > 98 % as determined by reducing SDS-PAGE.
• Bioactivity: Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is <0.7 ng/mL. The specific activity of recombinant human beta-NGF is > 1 x 10⁶ IU/mg.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 11 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.