Recombinant Human β-NGF/NGFB Protein (aa 122-239, Human Cells)

• Background:

  Human β-Nerve Growth Factor (β-NGF) was initially isolated in the mouse submandibular gland. It is composed of three non-covalently linked subunits α; β; and γ; it exhibits all the biological activities ascribed to NGF. It is structurally related to BDNF; NT-3 and NT-4 and belongs to the cysteine-knot family of growth factors that assume stable dimeric structures. Β-NGF is a neurotrophic factor that signals through its receptor β-NGF; and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. These results suggest that β-NGF is a pleiotropic cytokine; which in addition to its neurotropic activities may have an important role in the regulation of the immune system. Human β-NGF shares 90% sequence similarity with mouse protein and shows cross-species reactivity.

• Abbreviation:

  Β-NGF; NGFB

• Synonyms:

  Beta-NGF; Beta-Nerve Growth Factor; NGF; NGFB

• UniProt:

  P01138

• Accession Number:

  P01138

• Expression System:

  HEK293 Cells

• Tag:

  None

• Sequence:

  Ser122-Arg239

• Applications:

  Cell culture

• Field of Research:

  Neuroscience

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.04-0.4 ng/ml.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  13.3 kDa

• Observed Molecular Weight:

  14 kDa

• Species:

  Human