Recombinant Mouse β-NGF/NGFB Protein

• Background:

  NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4) . These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Mouse beta -NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90% homology at the amino acid level with human beta -NGF and 95.8% with rat beta -NGF. NGF signaling has been shown to play an important role in neuroprotection and repair. β-NGF acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival. It is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems.

• Abbreviation:

  β-NGF, NGFB

• Synonyms:

  Beta-NGF; Beta-nerve growth factor; Ngf

• UniProt:

  P01139

• Accession Number:

  P01139

• Expression System:

  E. coli

• Tag:

  C-His

• Sequence:

  Ser122-Gly241

• Applications:

  Cell culture

• Endotoxin:

  < 0.1 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 98 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is <1ng/mL. The specific activity of recombinant mouse beta-NGF is > 1 x 10⁶ IU/mg.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  14.4 kDa

• Observed Molecular Weight:

  11 - 17 kDa

• Formulation:

  Lyophilized from sterile 20 mM sodium citrate, 0.2 M NaCl, pH 4.5.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Mouse