NGF/NGFb ELISA Kit (Rat)

• Reproducibility:

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (pg/mL) 84 366 628 117 396 735 St. Dev. 4.62 16.84 37.68 6.9 20.2 52.92 %CV 5.5 4.60 6 5.89 5.10 7.2 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (pg/mL) 84 366 628 117 396 735 Mean (pg/mL) 84 366 628 117 396 735 St. Dev. 4.62 16.84 37.68 6.9 20.2 52.92 St. Dev. 4.62 16.84 37.68 6.9 20.2 52.92 %CV 5.5 4.60 6 5.89 5.10 7.2 %CV 5.5 4.60 6 5.89 5.10 7.2

• Gene Name:

  Nerve growth factor (beta polypeptide)

• Gene Aliases:

  Beta nerve growth factor, Beta NGF, HSAN5, MGC161426, MGC161428, Nerve growth factor (beta polypeptide), Nerve growth factor beta polypeptide, Nerve growth factor beta subunit, NGFB, NID67

• Gene ID:

  310738

• Accession Number:

  NP_001263984.1

• Reactivity:

  Rat

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: S122-G241

• Target:

  Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF.1 NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.2

• Type:

  ELISA Kit

• Applications:

  ELISA

• Assay Principle:

  Aviva's rat NGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for NGF has been precoated onto 96-well plates. Standards (NS0, S122-G241) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat NGF amount of sample captured in plate.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Cell culture supernates and serum

• Detection Range:

  15.6 pg/ml - 1,000 pg/ml

• Sensitivity:

  <1 pg/ml

• Reconstitution:

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• Specificity:

  Natural and recombinant rat NGF

• NCBI Gene Symbol:

  NGF

• Protein Name:

  Beta-nerve growth factor

• Gene Name URL:

  NGF

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001277055.1