Recombinant Mouse β-NGF/Beta-NGF Protein

Recombinant Mouse β-NGF/Beta-NGF Protein

• Background: Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. In addition, previous studies indicated that NGF may also have an important role in the regulation of the immune system.
• Synonyms: Ngfb
• CAS Number: 9000-83-3
• UniProt: P01139
• Accession Number: NP_001106168.1
• Host: CHO Stable Cells
• Origin Species: Mouse
• Tag: None
• Sequence: Ser 122-Gly 241
• Applications: Cell culture
• Purity: > 96 % as determined by reducing SDS-PAGE.
• Bioactivity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 1-8ng/mL.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 13.5 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.