Recombinant Human GM-CSF (CSF2)

• Description :

  GM-CSF is a hematopoietic growth factor that is produced by endothelial cells, monocytes, fibroblasts and T-lymphocytes following their activation by antigens or mitogens. GM-CSF stimulates the development of macrophages and neutrophils and plays a role in the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. Approximately 90% of the secreted colony stimulating activities are due to GM-CSF. While non-glycosylated and glycosylated GM-CSF show similar activities in vitro, fully glycosylated GM-CSF is biologically more active in vivo compared to its non-glycosylated counterpart. Human and murine GM-CSF are species-specific and therefore, exhibit no cross-reactivity. Recombinant Human GM-CSF is a non-glycosylated, monomeric protein that contains intra-chain disulfide bonds.

• Gene Name :

  CSF2

• Gene Aliases :

  GM-CSF, Granulocyte-macrophage colony-stimulating factor, CSF-2, Pluripoietin-alpha, Recombinant Human Granulocyte Macrophage Colony Stimulating Factor (CSF2)

• Gene ID :

  1437

• Accession Number :

  P04141

• Organism :

  Human

• Source :

  E. coli

• Concentration :

  < 1.0 EU/μg of recombinant protein as determined by the LAL method.

• Purity :

  >95% as determined by SDS-PAGE

• Solubility :

  A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

• Function :

  The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells was found to be ≤ 0.5 ng/mL

• Molecular Weight :

  15

• Shipping Conditions :

  Ambient Temperature

• Storage Conditions :

  The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

• Appearance :

  Lyophilized Powder

• Formulation :

  Lyophilized from a 0.2 μm filtered solution in Tris and NaCl

• CAS Number :

  9000-83-3