Recombinant Human GM-CSF (CSF2)

• Description:

  GM-CSF is a monomeric protein of 127 amino acids with two glycosylation sites. The protein is synthesized as a precursor of 144 amino acids, which included a hydrophobic secretory signal sequence at the amino terminal end. The sugar moiety is not required for the full spectrum of biological activities. Non-glycosylated and glycosylated GM-CSF show the same activities in vitro. Fully glycosylated GM-CSF is biologically more active in vivo than the non-glycosylated protein. This protein is secreted together with other factors by T cells and macrophages following cell activation by antigens or mitogens. Approximately 90% of the secreted colony stimulating activities are due to GM-CSF.

• Gene Name:

  CSF2

• Gene Aliases:

  GMCSF, Granulocyte-macrophage colony-stimulating factor, CSF-2, Molgramostin, Sargramostim, pluripoietin-alpha, Recombinant Human Granulocyte Macrophage Colony Stimulating Factor (CSF2)

• Gene ID:

  1437

• Accession Number:

  P04141

• Organism:

  Human

• Source:

  E. coli

• Concentration:

  <1.0 EU/μg of recombinant protein as determined by the LAL method.

• Purity:

  >95% as determined by SDS-PAGE

• Solubility:

  A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

• Function:

  The ED(50) as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 0.1 ng/mL, corresponding to a specific activity of ≥ 1.0 x 10^7 units/mg.

• Molecular Weight:

  15

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

• Appearance:

  Lyophilized Powder

• Formulation:

  Human GM-CSF was lyophilized from a 0.2 μm filtered solution in PBS, pH 7.0.

• CAS Number:

  9000-83-3