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Recombinant Rat Complement C4b/C4b protein (His tag)

CAT: 0763-PDMR100009-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0763-PDMR100009-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes.
Abbreviation
Complement C4b; C4b
Synonyms
C4; C4b; CO4; Complement C4; Complement C4 beta chain
UniProt
P08649
Accession Number
P08649
Expression System
HEK293 Cells
Tag
C-His
Sequence
Lys20–Ser673
Field of Research
Signal Transduction; immunology
Endotoxin
< 1.0 EU/mg of the protein as determined by the LAL method.
Purity
> 95% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
71.8 kDa
Observed Molecular Weight
70 kDa
Species
Rat

UniProtKB · P08649

Complement C4

CO4_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P08649
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
C4
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q62895, Q8R403
Protein keywords

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoproteinPhosphoproteinSulfationThioester bond

Biological process

Complement pathwayImmunityInflammatory responseInnate immunity

Technical term

Direct protein sequencingReference proteome

Cellular component

Secreted

Domain

Signal
Product Datasheet
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