Recombinant Human Complement Factor B/CFB Protein (His Tag)

• Background:

  Complement Factor B (CFB) belongs to the peptidase S1 family of enzymes. It is expressed by hepatocytes and macrophages and localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. CFB which is a component of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.

• Abbreviation:

  Complement Factor B; CFB

• Synonyms:

  BF; BFD; C3/C5 Convertase; CFB; Complement Factor B; GBG; Glycine-Rich Beta Glycoprotein; PBF2; Properdin Factor B

• UniProt:

  P00751

• Accession Number:

  AAH04143.1

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Sequence:

  Thr 26-Leu764

• Field of Research:

  Immunology

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Not Applicable

• Shipping Conditions:

  This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

• Storage Conditions:

  Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

• Calculated Molecular Weight:

  84.1 kDa

• Observed Molecular Weight:

  90-120 kDa

• Formulation:

  Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.

• Species:

  Human