Sucrose Fluorometric Assay Kit

• Type:

  Fluorometric method

• Field of Research:

  Glycolysis and Lipid Metabolism; Plant Physiology Series

• Assay Principle:

  Sucrose can be hydrolyzed by sucrase to produce glucose under acidic conditions, which is catalyzed by glucose oxidase to produce hydrogen peroxide. In the presence of HRP (horse radish peroxidase), hydrogen peroxide reacts with the fluorescent probe to form red fluorescent substance. The sucrose content can be calculated by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.

• Assay Performance Time:

  70 min

• Sample Type:

  Plant tissue

• Detection Range:

  0.15-15 μmol/L

• Sensitivity:

  0.15 μmol/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Inter CV:

  6.5%

• Intra CV:

  2.3%

• Detection Instrument:

  Fluorescence microplate reader