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Sucrose Fluorometric Assay Kit

CAT: 0763-E-BC-F042Size: 96 T (39 samples)Dry Ice: NoHazardous: No
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CAT#:0763-E-BC-F042Size:96 T (39 samples)
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Type
Fluorometric method
Field of Research
Glycolysis and Lipid Metabolism; Plant Physiology Series
Assay Principle
Sucrose can be hydrolyzed by sucrase to produce glucose under acidic conditions, which is catalyzed by glucose oxidase to produce hydrogen peroxide. In the presence of HRP (horse radish peroxidase), hydrogen peroxide reacts with the fluorescent probe to form red fluorescent substance. The sucrose content can be calculated by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.
Assay Performance Time
70 min
Sample Type
Plant tissue
Detection Range
0.15-15 μmol/L
Sensitivity
0.15 μmol/L
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Inter CV
6.5%
Intra CV
2.3%
Detection Instrument
Fluorescence microplate reader
Product Datasheet
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