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Glucose and Sucrose Colorimetric/Fluorometric Assay Kit

CAT: 0026-K616-100Size: 100 AssaysDry Ice: NoHazardous: No
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CAT#:0026-K616-100Size:100 Assays
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Glucose (C₆H₁₂O₆; FW: 180.16) and sucrose (C₁₂H₂₂O₁₁; FW:342.3) are the important fuel sources to generate universal energy molecule ATP. Measurement of glucose or sucrose level can be very important in both research and development process. Sucrose is a disaccharide which can be converted into one glucose and one fructose when adding Invertase. BioVision’s Glucose and Sucrose Assay Kit provides a convenient means for measuring glucose and sucrose levels from various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). To measure glucose level, glucose oxidase specifically oxidizes free-glucose generating a compound that reacts with the glucose probe to produce resorufin, which can be detected colorimetrically (O.D. 570 nm) or fluorometrically (Ex/Em 535/587). To measure sucrose, invertase can be added to the reaction to convert sucrose to free glucose and fructose, so total glucose level can be measured. Then the sucrose level = Total Glucose – Free Glucose.
Features & Benefits
• Simple procedure; takes ~40 minutes • Fast and convenient • Kit provides all necessary buffers and reagents for a accurate assay of glucose and sucrose individually in various biological samples
Shipping Conditions
gel pack
Storage Conditions
-20°C
Shelf Life
12 months

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