Lactulose Fluorometric Assay Kit

• Type:

  Fluorometric method

• Field of Research:

  Metabolic Diseases

• Assay Principle:

  Lactulose (4-o-β-galactopyranosyl-D-fructo-furanose), also known as galactoside fructose, 4-β-D-galactosid-D-fructose and lactulose, contains one molecule of galactose and one molecule of fructose. Lactulose is a kind of synthetic disaccharide formed by base isomerization of lactose catalyzed by free amino group of casein during milk heat treatment. Lactulose can produce a specific product under the action of enzyme, which reacts with the chromogenic agent to produce a fluorescence product. The excitation wavelength is 530 nm, and the emission wavelength is 590 nm.

• Assay Performance Time:

  150 min

• Sample Type:

  Dairy product; feces

• Detection Range:

  4.78-500 μmol/L

• Sensitivity:

  4.78 μmol/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Inter CV:

  7.4-9.7%

• Intra CV:

  2.6-3.8%

• Detection Instrument:

  Fluorescence Microplate Reader