Sucrose Colorimetric Assay Kit

CAT:

763-E-BC-K161-S-02

Size:

50 Assays

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Field of Research: Glycolysis and Lipid Metabolism
Detection Method: Colorimetric method
Assay Principle: Sucrose in plant tissue is hydrolyzed to glucose and fructose in boiling water bath under acidic conditions. 5-hydroxymethyl furfural was synthesized from fructose under acid condition and measure the ultraviolet absorption of 5-hydroxymethyl furfural. Glucose must be dissimilated into ketose structure and reduced to obtain 5-hydroxymethylfurfural, but the rate of isomerization of glucose to ketose is very slow. Therefore, the ultraviolet absorption of glucose is much smaller than fructose.
Assay Performance Time: 40 min
Detection Range: 0.32-70 μmol/mL
Sensitivity: 0.32 μmol/mL
Shipping Conditions: Ice bag
Storage Conditions: This product can be stored at 2~8°C for 12 months with shading light.

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