Sucrose Colorimetric Assay Kit

Sucrose Colorimetric Assay Kit

• Field of Research: Glycolysis and Lipid Metabolism
• Detection Method: Colorimetric method
• Assay Principle: Sucrose in plant tissue is hydrolyzed to glucose and fructose in boiling water bath under acidic conditions. 5-hydroxymethyl furfural was synthesized from fructose under acid condition and measure the ultraviolet absorption of 5-hydroxymethyl furfural. Glucose must be dissimilated into ketose structure and reduced to obtain 5-hydroxymethylfurfural, but the rate of isomerization of glucose to ketose is very slow. Therefore, the ultraviolet absorption of glucose is much smaller than fructose.
• Assay Performance Time: 40 min
• Detection Range: 0.32-70 μmol/mL
• Sensitivity: 0.32 μmol/mL
• Shipping Conditions: Ice bag
• Storage Conditions: This product can be stored at 2~8°C for 12 months with shading light.