Sucrose Colorimetric Assay Kit
CAT:
763-E-BC-K161-S-02
Size:
50 Assays
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No




Sucrose Colorimetric Assay Kit
- Field of Research: Glycolysis and Lipid Metabolism
- Detection Method: Colorimetric method
- Assay Principle: Sucrose in plant tissue is hydrolyzed to glucose and fructose in boiling water bath under acidic conditions. 5-hydroxymethyl furfural was synthesized from fructose under acid condition and measure the ultraviolet absorption of 5-hydroxymethyl furfural. Glucose must be dissimilated into ketose structure and reduced to obtain 5-hydroxymethylfurfural, but the rate of isomerization of glucose to ketose is very slow. Therefore, the ultraviolet absorption of glucose is much smaller than fructose.
- Assay Performance Time: 40 min
- Detection Range: 0.32-70 μmol/mL
- Sensitivity: 0.32 μmol/mL
- Shipping Conditions: Ice bag
- Storage Conditions: This product can be stored at 2~8°C for 12 months with shading light.