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Rat ApoE (Apolipoprotein E) CLIA Kit

CAT: 0763-E-CL-R0718-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-CL-R0718-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
ApoE
UniProt
P02650
Cross Reactivity
This kit recognizes Rat ApoE in samples.No significant cross-reactivity or interference between Rat ApoE and analogues was observed
Target
Apo-E; MGC1571; apolipoprotein E3; apolipoprotein E
Type
Sandwich-ELISA
Applications
CLIA
Field of Research
Cancer; Cardiovascular; Metabolism; Developmental biology; Neuroscience; Signal transduction; Stem cells
Assay Principle
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ApoE. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ApoE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ApoE, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat ApoE. You can calculate the concentration of Rat ApoE in the samples by comparing the RLU value of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.63-40 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
0.38 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C/-20°C,6 months
Species
Rat
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Rat ApoE concentrations in serum, plasma and other biological fluids.

UniProtKB · P02650

Apolipoprotein E

APOE_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P02650
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Apoe
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
Apo-E
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Cellular component

ChylomicronEndosomeExtracellular matrixHDLSecretedVLDL

Technical term

Direct protein sequencingReference proteome

PTM

GlycoproteinOxidationPhosphoprotein

Molecular function

Heparin-binding

Biological process

Lipid transportTransport

Ligand

Lipid-binding

Domain

RepeatSignal