Mouse ApoE (Apolipoprotein E) ELISA Kit

ApoE

P08226

This kit recognizes Mouse ApoE in samples. No significant cross-reactivity or interference between Mouse ApoE and analogues was observed

Apo-E; MGC1571; apolipoprotein E3

Sandwich-ELISA

ELISA

Cancer; Cardiovascular; Metabolism; Developmental biology; Neuroscience; Signal transduction; Stem cells

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ApoE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ApoE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ApoE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ApoE. You can calculate the concentration of Mouse ApoE in the samples by comparing the OD of the samples to the standard curve.

3 h 30 min

Serum, plasma and other biological fluids

1.56-100 ng/mL

Both intra-CV and inter-CV are < 10%.

80%-120%

0.94 ng/mL

Ice bag

2-8°C,12 months

https://pubmed.ncbi.nlm.nih.gov/36425057/

https://onlinelibrary.wiley.com/doi/abs/10.1002/brb3.2702

https://www.spandidos-publications.com/10.3892/mmr.2020.11131

https://onlinelibrary.wiley.com/doi/abs/10.1002/jdn.10041

Mouse