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CLIA Kit for Apolipoprotein E (APOE)

CAT: 0338-TRV-047650-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0338-TRV-047650-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
Apo-E; AD2; Apoprotein; Alzheimer Disease 2 (E4-Associated, Late Onset
UniProt
P02649
Applications
Chemiluminescent immunoassay for Antigen Detection.
Field of Research
Metabolic pathway; Cardiovascular biology; Hepatology
Assay Principle
The microplate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein E (APOE) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Apolipoprotein E (APOE) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Apolipoprotein E (APOE) level in the sample or standard.
Assay Protocol
1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 100µ L Substrate Solution. Incubate 10 minutes at 37° C;
8. Read RLU value immediately.
Assay Performance Time
2h, 40min
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.82-600ng/mL
Precision
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Apolipoprotein E (APOE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Apolipoprotein E (APOE) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV
Sensitivity
The minimum detectable dose of this kit is typically less than 0.37ng/mL
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Format
48T, 96T, 96Tx5, 96Tx10, 96Tx100
Specificity
This assay has high sensitivity and excellent specificity for detection of Apolipoprotein E (APOE) . No significant cross-reactivity or interference between Apolipoprotein E (APOE) and analogues was observed.
Product Name Synonym
Apolipoprotein E
Method
Double-antibody Sandwich
Organism Species
Homo sapiens (Human)

UniProtKB · P02649

Apolipoprotein E

APOE_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P02649
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
APOE
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
Apo-E
EC number
—
Processing
Precursor
Secondary accessions
B2RC15, C0JYY5, Q9P2S4
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Disease

Alzheimer diseaseAmyloidosisDisease variantHyperlipidemiaNeurodegeneration

Biological process

Cholesterol metabolismHost-virus interactionLipid metabolismLipid transportSteroid metabolismSterol metabolismTransport

Cellular component

ChylomicronEndosomeExtracellular matrixHDLSecretedVLDL

PTM

GlycationGlycoproteinOxidationPhosphoprotein

Molecular function

Heparin-binding

Ligand

Lipid-binding

Domain

RepeatSignal

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