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Human ApoE (Apolipoprotein E) ELISA Kit

CAT: 0763-E-EL-H0470-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-EL-H0470-01Size:24 Tests
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Abbreviation
ApoE
UniProt
P02649
Cross Reactivity
This kit recognizes Human ApoE in samples. No significant cross-reactivity or interference between Human ApoE and analogues was observed
Target
Apo-E; MGC1571; apolipoprotein E3
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Cardiovascular; Metabolism; Developmental biology; Neuroscience; Signal transduction; Stem cells
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ApoE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ApoE. You can calculate the concentration of Human ApoE in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
23.44-1500 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
14.06 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.sciencedirect.com/science/article/pii/S0896627321000052
Citation 02
https://www.mdpi.com/2098480
Citation 03
https://www.frontiersin.org/articles/10.3389/fninf.2021.662967/full
Citation 04
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6650621/
Citation 05
https://www.karger.com/Article/Abstract/520864
Detection Instrument
Microplate reader
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human ApoE concentrations in serum, plasma and other biological fluids.

UniProtKB · P02649

Apolipoprotein E

APOE_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P02649
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
APOE
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
Apo-E
EC number
—
Processing
Precursor
Secondary accessions
B2RC15, C0JYY5, Q9P2S4
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Disease

Alzheimer diseaseAmyloidosisDisease variantHyperlipidemiaNeurodegeneration

Biological process

Cholesterol metabolismHost-virus interactionLipid metabolismLipid transportSteroid metabolismSterol metabolismTransport

Cellular component

ChylomicronEndosomeExtracellular matrixHDLSecretedVLDL

PTM

GlycationGlycoproteinOxidationPhosphoprotein

Molecular function

Heparin-binding

Ligand

Lipid-binding

Domain

RepeatSignal

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