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RecombinantEGF, Human

CAT: 0013-GTR18991891-01Size: 1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18991891-01Size:1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Recombinant human Epidermal Growth Factor (EGF) is a 6,200 Da protein containing 53 amino acid residues.
Product Name Alternative
Epidermal Growth Factor
Source
Escherichia coli.
Endotoxin
Less than 0.1ng/μg (1 EU/μg) of recombinant human Epidermal Growth Factor (EGF) as determined by LAL test.
Purity
Greater than 95% as determined by (a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel
Bioactivity
The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less then 2 ng/ml, corresponding to a specific activity of 5.0x10ˆ5 IU/ mg.
Form
Recombinant human Epidermal Growth Factor (EGF) was lyophilized after extensive dialysis against 10mM Phosphate buffer, pH 7.0, 200mM NaCl buffer.
Molecular Weight
6.0 kDa+/-10% determined by reduced SDS-PAGE
Storage Conditions
Lyophilized recombinant Human Epidermal Growth Factor (rhEGF) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhEGF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.
Notes
For research use only.
Applications Notes
It is recommended to reconstitute the lyophilized recombinant human Epidermal Growth Factor (EGF) in sterile 18 MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions.
Preservative
Recombinant human Epidermal Growth Factor (EGF) was lyophilized after extensive dialysis against 10mM Phosphate buffer, pH 7.0, 200mM NaCl buffer.
AA Sequence
MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR

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