RecombinantEGF, Rat

• Description:

  Epidermal Growth Factor (EGF) is a cytokine with 53 amino acids, originally found in mouse submaxillary gland. EGF binds to EGF receptors, ErbB1 and B4, and causes them to be dimerized and phosphorylated. The dimerized and phosphorylated EGFR can bind to several intracellular targets, such as phospholipase Cγ and Ras-GTPase-acting protein, and achieve a series of cascade reactions. EGF is involved in the regulation of cell proliferation and differentiation, and is up-regulated during wound healing, accelerating reepitheliazation and increasing tensile strength. It also stimulates neurite outgrowth and increases the uptake of dopamine in the central nervous system. On the other hand, EGF is up-regulated in the glioma cancer, and related to the length of survivals of the patients.Recombinant rat Epidermal Growth Factor (rrEGF) produced in E.coli is a single non-glycosylated polypeptide chain containing 54 amino acids. A fully biologically active molecule, rrEGF has a molecular mass of 6.3 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

• Endotoxin:

  < 0.2 EU/μg, determined by LAL method.

• Purity:

  > 95% by SDS-PAGE analysis.

• Bioactivity:

  ED50 < 0.08 ng/mL, measured by a cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 1.25x 10ˆ7 units/mg.

• Reconstitution:

  Reconstituted in ddH2O at 100 μg/mL.

• Molecular Weight:

  6.3 kDa, observed by reducing SDS-PAGE.

• Storage Conditions:

  Lyophilized recombinant rat Epidermal Growth Factor (rrEGF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrEGF should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.

• Appearance:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Formulation:

  Lyophilized after extensive dialysis against PBS.

• Host or Source:

  Escherichia coli.

• Recommended Usage:

  This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

• CAS Number:

  9000-83-3

• AA Sequence:

  MNSNTGCPP SYDGYCLNGG VCMYVESVDR YVCNCVIGYI GERCQHRDLR WWKLR