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Mouse Cotinine (Cot) ELISA Kit

CAT: 0013-GTR18873315-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873315-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mouse Cotinine (Cot) ELISA Kit
Product Name Alternative
Cotinine
Reactivity
Mouse
Target
Cot
Field of Research
Pharmacology & Drug Discovery
Assay Type
Competitive
Assay Performance Time
2.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.62 ng/mL
Notes
For research use only.
Applications Notes
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse Cot. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Cot. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Cot in the samples is then determined by comparing the OD of the samples to the standard curve.
Tested Applications
ELISA
Dynamic Range
1.57-100 ng/mL

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