Mouse Cot (Cotinine) ELISA Kit

• Product Name Alternative:

  Cotinine

• Reactivity:

  Mouse

• Field of Research:

  Signal transduction

• Assay Type:

  Competitive Inhibition

• Assay Principle:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse Cot. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Cot. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Cot in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  2.5 H

• Standard:

  100 ng/mL

• Sample Type:

  Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• Detection Range:

  1.57 - 100 ng/mL

• Sensitivity:

  0.62 ng/mL