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Zebrafish Cot (Cotinine) ELISA Kit

CAT: 0855-ELK11344-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK11344-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
Cotinine
Reactivity
Zebrafish
Field of Research
Signal transduction
Assay Type
Competitive Inhibition
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish Cot. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish Cot. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish Cot in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
2h
Standard
100 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
1.57-100 ng/mL
Sensitivity
0.61 ng/mL

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