TIE-2

• Description :

  Tie-1/Tie and Tie-2/Tek are receptor tyrosine kinases with unique structural characteristics including two immunoglobulin-like domains flanking three epidermal growth factor (EGF) -like domains, followed by three fibronectin type III-like repeats in the extracellular region, and a split tyrosine kinase domain in the cytoplasmic region. Tie-2 is involved in vascular stabilization and remodeling. Although less well understood, Tie-1 may also act as an ANG receptor, possibly in complex with Tie-2. Human Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Tie-2 is a receptor for the angiopoietin (ANG) family: ANG-1, ANG-2, and ANG-3 (mouse) /-4 (human) . Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.

• Synonyms :

  TEK; TIE2; VMCM; TIE-2; VMCM1; CD202B

• NCBI Gene ID :

  7010

• UniProt :

  Q02763

• Accession Number :

  NP_000450.2

• Accession Number mRNA :

  NM_000459.3

• Chromosomal Location :

  9p21

• Reactivity :

  Anti-Human

• Cross Reactivity :

  Human

• Target Antigen :

  Recombinant human soluble extracellular TIE-2

• Clone :

  (#tek16)

• Applications :

  ELISA, WB, FC

• Purification Method :

  Protein G chromatography

• Assay Protocol :

  Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

• Bioactivity :

  ELISA: Use at 1-2 µg/ml. Western blotting: Use at 2-5 µg/ml. FACS analysis: Use at 2-5 µg/ml.

• Form :

  Lyophilized

• Buffer :

  PBS

• Reconstitution :

  Water

• Storage Conditions :

  The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

• Host or Source :

  Mouse

• Isotype :

  IgG1