TIE-1

• Description:

  Tie-1/Tie and Tie-2/Tek are receptor tyrosine kinases with unique structural characteristics including two immunoglobulin-like domains flanking three epidermal growth factor (EGF) -like domains, followed by three fibronectin type III-like repeats in the extracellular region, and a split tyrosine kinase domain in the cytoplasmic region. Tie-2 is involved in vascular stabilization and remodeling. Although less well understood, Tie-1 may also act as an ANG receptor, possibly in complex with Tie-2. Human Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Tie-2 is a receptor for the angiopoietin (ANG) family: ANG-1, ANG-2, and ANG-3 (mouse) /-4 (human) . Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.

• Synonyms:

  TIE1; TIE; JTK14

• NCBI Gene ID:

  7075

• UniProt:

  P35590

• Accession Number:

  NP_005415

• Accession Number mRNA:

  NM_005424

• Chromosomal Location:

  1p34-p33

• Reactivity:

  Anti-Human

• Cross Reactivity:

  Human

• Target Antigen:

  Recombinant human soluble extracellular TIE-1

• Clone:

  (#6F12)

• Label:

  Biotin

• Applications:

  ELISA

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

• Bioactivity:

  ELISA: Use at 1-15 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Additives:

  BSA (50x), 0.02% sodium azide

• Reconstitution:

  Water

• Storage Conditions:

  The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

• Notes:

  The unconjugated antibody will detect native human TIE-1 in ELISA experiments and on the surface of different human cell types. The antibody can be used for ELISA experiments and Western blotting.

• Host or Source:

  Mouse

• Isotype:

  IgG1